What is the Difference Between Anthrone and Molisch Test?

The Anthrone and Molisch tests are both analytical tests used to detect carbohydrates in a given solution. However, there are differences between the two tests:

1. Indicators: The Anthrone test uses Anthrone reagent, which forms a green-colored complex when carbohydrates are present. On the other hand, the Molisch test uses Molisch's reagent and forms a violet layer to indicate the presence of carbohydrates.
2. Quantification: The intensity of the color in the Anthrone test increases with the increase in the amount of carbohydrates. In contrast, the Molisch test is semi-quantitative, meaning it can only detect the presence of carbohydrates but not quantify their concentration.
3. Procedure: Both tests involve mixing the test solution with a small amount of the respective reagent (Anthrone or Molisch) and adding a small amount of sulfuric acid. However, the Anthrone test involves boiling the solution in a water bath for 10 minutes before analyzing the color formation.

In summary, the main differences between the Anthrone and Molisch tests are the indicators used, the quantification capabilities, and the procedural differences in the tests.

Comparative Table: Anthrone vs Molisch Test

The Anthrone and Molisch tests are both used to detect carbohydrates in a given solution, but they have different principles and reagents. Here is a comparison between the two tests:

In summary, both the Anthrone and Molisch tests are used to detect carbohydrates in solutions, but they have different principles and reagents. The Anthrone test uses an Anthrone reagent and detects the presence of carbohydrates through the formation of a blue-green color, while the Molisch test uses Molisch's reagent and sulfuric acid to detect carbohydrates by forming a purple or purplish-red ring.