What is the Difference Between Competitive and Noncompetitive ELISA?
🆚 Go to Comparative Table 🆚The difference between competitive and noncompetitive ELISA lies in the reaction method used and the presence or absence of antigen competition. Here are the main differences between the two:
- Competitive ELISA:
- Also known as inhibition ELISA or competitive immunoassay.
- Measures the concentration of an antigen by detecting signal interference.
- Relies on the competition between the antigen of interest and a reference antigen.
- More suitable for measuring low amounts of antigen of interest.
- Can be more complex and require an inhibitor antigen.
- Ideal for small antigens.
- Noncompetitive ELISA:
- Does not rely on the competition between target antigens and reference antigens.
- Produces a signal directly, without the need for competition.
- Does not depend on the number of antigens present in a sample.
- Generally simpler and more sensitive than competitive ELISA.
In summary, competitive ELISA measures the concentration of an antigen by detecting the interference of signal due to the competition between the antigen of interest and a reference antigen, while noncompetitive ELISA directly measures the signal produced without relying on antigen competition.
Comparative Table: Competitive vs Noncompetitive ELISA
Competitive and noncompetitive ELISA are two major types of enzyme-linked immunosorbent assays (ELISA) that differ in their methodology and application. Here is a table summarizing the differences between them:
Parameter | Competitive ELISA | Noncompetitive ELISA |
---|---|---|
Definition | Measures the concentration of an analyte through competition between the analyte and a reference antigen. | Measures the analyte-bound sites. |
Sensitivity | Less sensitive. | More sensitive. |
Measurement | Measures analyte-unbound sites. | Measures analyte-bound sites. |
Excess Antibody | Does not use excess antibodies. | Uses excess antibodies. |
Signal Generating Group | Another antigen. | An antibody. |
Precision and Working Range | Less precise and has a narrower working range. | More precise and has a wider working range. |
Competitive ELISA relies on the competition between the antigen of interest and a reference antigen, making it also known as an inhibition ELISA. It is more suitable for measuring low amounts of antigen and is a sensitive method. On the other hand, noncompetitive ELISA does not depend on the number of antigens present in a sample and does not involve competition between target antigens and reference antigens.
- Sandwich Elisa vs Competitive Elisa
- Competitive vs Noncompetitive Inhibition
- Direct vs Indirect ELISA
- RIA vs ELISA
- ELISA vs Rapid Test
- Elisa vs Western Blot
- Non-Competitive vs Allosteric Inhibition
- Elisa vs Elispot
- Allosteric vs Non-allosteric Enzymes
- Enzymatic vs Nonenzymatic Reaction
- Neutralizing vs Binding Antibodies
- Monoclonal Antibodies vs Polyclonal Antibodies
- Serum Protein Electrophoresis vs Immunofixation
- Complete vs Incomplete Antibodies
- Antigen vs Antibody Test
- Antiserum vs Antibody
- Immunoglobulin vs Antibody
- Immunofluorescence vs Immunohistochemistry
- Catalytic vs Non Catalytic Reaction