What is the Difference Between DMEM and EMEM?
🆚 Go to Comparative Table 🆚DMEM (Dulbecco's Modified Eagle Medium) and EMEM (Eagle's Minimum Essential Medium) are two popular animal cell culture media that mainly differ in their nutrient compositions. The key differences between DMEM and EMEM include:
- Nutrient Composition: EMEM is a minimal media and contains all the essential factors required for the successful growth of cells. In contrast, DMEM is a modified form of EMEM, where the nutrient concentrations are increased along with the addition of some new components.
- Amino Acids, Vitamins, and Inorganic Phosphate: DMEM has lower concentrations of amino acids, vitamins, and inorganic phosphate compared to MEM (Minimum Essential Medium), which is the parent medium of EMEM.
- Lactate and Glutamine: DMEM includes lactate and glutamine in its composition, while EMEM does not.
- Growth Rate: Cells will grow faster and deplete the medium slower in DMEM due to its increased nutrient concentrations.
Despite these differences, both media types share some similarities:
- Both media types are liquid formulations.
- Both media types are modified media from the basal media.
- Both media types contain the essential amino acids, vitamins, and inorganic salts that are required for growth.
- Both media types are incomplete, and serum should be added for cell growth.
- Both media types use glucose as their carbon source.
- Both media types have a higher pH and are adjusted by adding sodium bicarbonate.
In summary, DMEM and EMEM are both cell culture media with different nutrient compositions. DMEM is a modified, more complex medium with higher concentrations of amino acids, vitamins, and additional components like lactate and glutamine, while EMEM is a minimal media with the essential nutrients required for cell growth.
Comparative Table: DMEM vs EMEM
Here is a table summarizing the differences between DMEM and EMEM:
Feature | DMEM (Dulbecco's Modified Eagle Medium) | EMEM (Eagle's Minimum Essential Medium) |
---|---|---|
pH | 7.2 +/- 0.2 | 0.0 +/- 0.2 |
osmolarity | 310 mOsm/L | 310 mOsm/L |
sodium bicarbonate | 1250 mg/L | 1220 mg/L |
glucose | 1000 mg/L | 1000 mg/L |
sodium pyruvate | Yes | No |
HEPES | Yes | No |
vitamins | 5000 IU penicillin and 5000 ug streptomycin per 100 ml of medium | 5000 IU penicillin and 5000 ug streptomycin per 100 ml of medium |
usage | for a wide variety of cells, including MDCK, HeLa, HEK293, HT-1080, MCF-7, and primary cells for cell culture | suitable for most types of cells |
Both DMEM and EMEM are Classical Media, originally formulated in the 1950s by Harry Eagle at the NIH. DMEM is widely used for a variety of cells, including MDCK, HeLa, HEK293, HT-1080, MCF-7, and primary cells for cell culture. EMEM is suitable for most types of cells. The differences highlighted in the table above have implications for cell growth and viability, so it is essential to select the appropriate medium based on cell type and experimental requirements.
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