What is the Difference Between HFR and F+ Strains?
🆚 Go to Comparative Table 🆚The main difference between HFR and F+ strains lies in the structure and location of the F plasmid within the bacterial cells:
- F+ Strains: These bacterial cells possess an F plasmid that is separate from the bacterial chromosome, and it exists independently in the cytoplasm. The F plasmid contains fertility factor coding genes, and bacteria with F plasmids are capable of forming sex pili and transferring plasmids to other bacteria.
- HFR Strains: These bacterial cells have an F plasmid integrated into their chromosomes, forming recombinant DNA. The F plasmid is no longer separate from the bacterial chromosome but is integrated into it. HFR strains can transfer not only the F plasmid but also a small portion of the bacterial chromosome during conjugation.
In summary, F+ strains have an independent F plasmid in their cytoplasm, while HFR strains have the F plasmid integrated into their bacterial chromosomes.
Comparative Table: HFR vs F+ Strains
The main difference between HFR and F+ strains lies in the integration of the F plasmid into the bacterial chromosome. Here is a table comparing the two strains:
Feature | F+ Strains | HFR Strains |
---|---|---|
F Plasmid | Free in the cytoplasm | Integrated into the bacterial chromosome |
Fertility | Independent of the chromosomal DNA | Dependent on the chromosomal DNA |
Gene Transfer | Can transfer F plasmid to other bacteria | Transfers a small portion of the integrated F plasmid during conjugation |
Bacterial Conjugation | Participates in bacterial conjugation | Participates in bacterial conjugation, but with a lower frequency of gene transfer |
In summary, F+ strains have an independent F plasmid free in the cytoplasm, while HFR strains have the F plasmid integrated into their bacterial chromosomes.
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