What is the Difference Between Hypersil and Inertsil Column?

Hypersil and Inertsil are trade names for two types of reverse-phase HPLC columns used in chromatography. The key difference between them lies in the hydroxyl (OH) groups present in their structures.

Hypersil column, also known as BDS column, is a type of reverse-phase HPLC column with blocked OH groups. In other words, the hydroxyl groups in this column are deactivated or not free. Hypersil columns are often packed with octadecasilane chains.

On the other hand, Inertsil column, also known as ODS column, is a type of reverse-phase HPLC column with free OH groups. These columns contain functional groups that are unrestricted and can interact with the solute molecules in the mobile phase, leading to higher peak tailing.

In summary, the main differences between Hypersil and Inertsil columns are:

• Hypersil columns have blocked OH groups, while Inertsil columns have free OH groups.
• Hypersil columns are often packed with octadecasilane chains, while Inertsil columns have bulky ODS groups bonded to the silanol through chemical reactions.

Both columns are used in reverse-phase chromatography, but they have different separating capabilities and peak tailing characteristics. It is essential to choose the appropriate column based on the specific requirements of the analysis.

Comparative Table: Hypersil vs Inertsil Column

Hypersil and Inertsil are trade names for reverse-phase HPLC columns. The key difference between Hypersil and Inertsil columns is that Hypersil columns have blocked -OH groups, while Inertsil columns contain free -OH groups. Here is a comparison table highlighting the differences between Hypersil and Inertsil columns:

Both Hypersil and Inertsil columns are widely used in HPLC analysis, but their performance may vary depending on the specific column type and operating conditions.