What is the Difference Between Isoschizomers and Isocaudomers?

Isoschizomers and isocaudomers are both types of restriction enzymes, but they differ in their recognition and cleavage properties. Here are the key differences between them:

Isoschizomers:

• Recognize the same DNA sequence and cleave at the same restriction site.
• Have the same recognition site and produce compatible ends.
• Examples include SphI and BbuI, which recognize and cleave at CGTAC/G, and HpaII and MspI, which both recognize the sequence 5′-CCGG-3′.

Isocaudomers:

• Produce the same nucleotide extensions but have different recognition sites.
• Example: NcoI from Nocardia and Sau3AI from Staphylococcus.

Both isoschizomers and isocaudomers are prokaryotic in origin and have a restriction recognition site as well as a digestion site. They are useful tools in recombinant DNA technology, which involves the manipulation of DNA to create new combinations of genetic material.

Comparative Table: Isoschizomers vs Isocaudomers

Isoschizomers and isocaudomers are two types of restriction enzymes that differ in their recognition sequences and cleavage sites. Here is a table comparing their characteristics:

Isoschizomers are restriction enzymes that recognize the same sequence and cleave DNA at the same site, producing matching sticky ends. On the other hand, isocaudomers are restriction enzymes that recognize different sequences but produce the same ends, allowing them to be ligated to one another.