What is the Difference Between pBR322 and pUC19?
🆚 Go to Comparative Table 🆚pBR322 and pUC19 are both popular plasmids used as E. Coli cloning vectors, but they have some differences:
- Size: pBR322 is 4361 base pairs in length, while pUC19 is 2686 base pairs in length.
- Replication Rate: Due to its larger size, pBR322 has a slower rate of replication compared to pUC19.
- Origin of Replication: pBR322 has two origins of replication, one derived from the ColE1 plasmid and another from the pMB1 plasmid, whereas pUC19 has a single origin of replication derived from the pMB1 plasmid.
- Copy Number: pBR322 is a low copy number plasmid, while pUC19 is a high copy number plasmid.
- Antibiotic Resistance Genes: Both plasmids have an ampicillin resistance gene (AmpR) as a selectable marker. pBR322 also carries the tetracycline resistance gene (tetR).
- Lambda Genes: pUC19 has two Lambda genes (bla and lacZ), which encode for AmpR and β-galactosidase, respectively.
Both plasmids are used for molecular cloning and manipulation, but pUC19 is more commonly used due to its high copy number and faster replication rate.
Comparative Table: pBR322 vs pUC19
Here is a table summarizing the differences between pBR322 and pUC19:
Feature | pBR322 | pUC19 |
---|---|---|
Length | 4361 base pairs | 2686 base pairs |
Copy Number | Low copy number | High copy number |
Molecular Weight | 2.83 x 10^6 daltons | 1.75 x 10^6 daltons |
Replication Rate | Slower | Faster |
Restriction Sites | HindⅢ, BamHⅠ, SalⅠ, PstⅠ, PvuⅡ, PvuⅠ, ClaⅠ, and EcoRⅠ | HindⅢ, BamHⅠ, SalⅠ, SmaⅠ, SacⅠ, KpnⅠ, XbaⅠ, PstⅠ, SphⅠ, and EcoRⅠ |
Both pBR322 and pUC19 are popular plasmids used as E.Coli cloning vectors, with multiple restriction sites and an origin of replication (ori). pBR322 is a low copy number plasmid vector, while pUC19 is a high copy number plasmid vector. The difference in plasmid size affects the plasmid copy number, with larger plasmids tending to have a lower copy number.
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