What is the Difference Between RNASE A and RNASE H?
🆚 Go to Comparative Table 🆚The main difference between RNase A and RNase H lies in their mode of action and specificity. Here are the key differences:
- Specificity: RNase A is a pancreatic ribonuclease that specifically cleaves the 3' end of unpaired cytosine and uracil residues in single-stranded RNA. On the other hand, RNase H is a nonspecific ribonuclease enzyme that degrades RNA in RNA-DNA hybrids via hydrolytic reactions.
- Nature of RNA Cleaving: RNase A cleaves single-stranded RNA at higher salt concentrations, while RNase H cleaves RNA in RNA-DNA hybrids.
- Co-factors for Activity: RNase A does not need cofactors for its activity, whereas RNase H requires metal ions as cofactors for its function.
Both RNase A and RNase H are endoribonucleases, but they have distinct roles and mechanisms of action. RNase A is mainly used for breaking down RNA molecules, while RNase H is involved in the degradation of RNA in RNA-DNA hybrids, making it useful for removing poly-A tails from mRNA and other applications.
Comparative Table: RNASE A vs RNASE H
Here is a table comparing the differences between RNase A and RNase H:
Characteristic | RNase A | RNase H |
---|---|---|
Specificity | Hydrolizes phosphodiester bonds on the 3' side of pyrimidic nucleotides C and U of single-stranded RNA. | Hydrolizes the RNA portion of RNA/DNA hybrids and heteroduplexes between chimeric RNA-DNA strands and DNA non-specifically. |
Salt Concentration | Not affected by salt concentration. | Works at a higher salt concentration (0.3M or higher NaCl concentration). |
Applications | Not used to remove "poly-A tail" in mRNA hybridized to oligo (dt). | Used to remove "poly-A tail" in mRNA hybridized to oligo (dt). |
Additional Functions | Used in nuclease protection assays. | Involved in RNA template removal during complementary DNA (C-DNA) synthesis. |
RNase A is a popular enzyme in molecular research and is extracted from bovine pancreas. It is an endoribonuclease that hydrolyzes phosphodiester bonds on the 3' side of pyrimidic nucleotides C and U of single-stranded RNA. On the other hand, RNase H is a nonspecific ribonuclease enzyme that degrades RNA in RNA-DNA hybrids via hydrolytic reactions. It is involved in removing poly-A tails from mRNA hybridized to oligo (dt) and is used in RNA template removal during complementary DNA synthesis.
- hnRNA vs mRNA
- Benzonase vs DNase
- RNA Polymerase Core vs Holoenzyme
- Hemagglutinin vs Neuraminidase
- Protease vs Proteinase
- Endonuclease vs Exonuclease
- Hydrolase vs Transferase
- DNA vs RNA Extraction
- Proteinase K vs Protease
- Ribozymes vs Protein Enzymes
- DNA vs DNAse
- Restriction Endonuclease vs Exonuclease
- DNA Polymerase vs RNA Polymerase
- CRISPR vs RNAi
- RNA vs mRNA
- Renin vs Rennin
- DNA vs RNA
- Holoenzyme vs Apoenzyme
- DNA vs RNA Isolation