What is the Difference Between Selectable Marker and Reporter Gene?
🆚 Go to Comparative Table 🆚The main difference between a selectable marker and a reporter gene lies in their functions in genetic engineering and molecular biology. Here are the key differences between the two:
- Selectable Marker: These are gene sequences that help distinguish between transformed and non-transformed cells. They are used to select and identify genetically modified cells, and they often provide a selective advantage by conferring resistance to toxic substances. Examples of selectable markers include antibiotic resistance genes and herbicide resistance genes.
- Reporter Gene: These are test sequences whose expression can be easily monitored and measured. They are used to tag another gene or a promoter and measure the level of expression of the transformed gene. Reporter genes are often fluorescent in nature, providing visual cues for estimation. Examples of reporter genes include luciferase, β-galactosidase, and green fluorescent protein (GFP).
In summary, selectable markers are used for the selection and identification of genetically modified cells, while reporter genes are used for the visualization and measurement of gene expression patterns. Both types of genetic elements are indispensable tools in molecular biology and genetic research, each serving a distinct purpose.
Comparative Table: Selectable Marker vs Reporter Gene
Here is a table comparing the differences between selectable markers and reporter genes:
| Feature | Selectable Marker Genes | Reporter Genes |
|---|---|---|
| Purpose | Differentiate between transformed and non-transformed cells | Measure the level of expression of a gene or promoter |
| Function | Confer resistance to toxicity in the transformed cells, allowing them to survive in the presence of a selective agent | Provide visual cues or quantifiable signals to assess the expression of transformed genes |
| Examples | Antibiotic resistance genes, herbicide resistance genes | Fluorescent proteins (e.g., GFP), luciferase, β-galactosidase |
| Detection | Selective growth on medium containing a specific selective agent (e.g., herbicide or antimetabolite) | Quantification of protein production or fluorescence |
Selectable marker genes are used to differentiate between transformed and non-transformed cells, typically by conferring resistance to a selective agent such as an antibiotic or herbicide. In contrast, reporter genes are used to measure the level of expression of a gene or promoter, often by producing a fluorescent protein or other quantifiable signal.
- Transgenesis vs Selective Breeding
- GMO vs Selective Breeding
- Selective Breeding vs Genetic Engineering
- Reporter vs Journalist
- Correspondent vs Reporter
- Gene vs Trait
- Transgenic vs Knockout Mice
- Pseudogene vs Gene
- Gene vs Genome
- Oncogene vs Tumor Suppressor Gene
- Gene Mapping vs Gene Sequencing
- Gene Addition vs Gene Replacement
- Candidate Gene vs GWAS
- Enhancer vs Promoter
- Artificial Selection vs Genetic Engineering
- Gene Knockout vs Knockdown
- Gene Expression vs Gene Regulation
- Genetic Engineering vs Genome Editing
- Genetic Map vs Linkage Map