What is the Difference Between Callus Culture and Suspension Culture?
🆚 Go to Comparative Table 🆚The main difference between callus culture and suspension culture lies in the structure and growth medium of the cells. Here are the key differences:
Callus Culture:
- Callus culture is an undifferentiated, unorganized mass of dividing cells grown on an agar medium.
- It is used in plant tissue culture to regenerate plants and amplify limiting plant material.
- Cells in callus culture are grown on a solid nutrient medium.
Suspension Culture:
- Suspension culture is a liquid culture in which single cells or small aggregates of cells multiply while suspended in an agitated liquid medium.
- It is used for large-scale production of cells and biomolecules.
- Cells in suspension culture are grown in a liquid medium.
Both culturing techniques are types of cell cultures grown in laboratories for various purposes, such as studying cell growth, development, and biological processes. They are sub-culturing techniques and are in vitro, meaning they are performed outside of a living organism. Both cultures require a good nutrient supply and continuous monitoring of the process until growth is completed.
Comparative Table: Callus Culture vs Suspension Culture
Based on the information provided, the differences between callus culture and suspension culture are as follows:
Feature | Callus Culture | Suspension Culture |
---|---|---|
Cell Form | Undifferentiated, unorganized mass of dividing cells | Single cells and small groups of cells suspended in a liquid medium |
Medium | Solid nutrient medium (agar) | Liquid nutrient medium |
Cell Morphology | Irregular shape | Single or groups of cells |
Aeration | No continuous agitation required | Continuous agitation required to mix the content and aerate |
Application in Plant Tissue Culture | Regeneration of plants and amplification of limiting plant material | Not commonly used in plant tissue culture |
Callus culture is an undifferentiated, unorganized mass of dividing cells grown on an agar medium, while suspension culture is a liquid culture consisting of single cells and small groups of cells suspended in a liquid medium. Growth conditions and nutrient levels should be maintained and continuously monitored until the growth is completed for both cultures.
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