What is the Difference Between PFK-1 and PFK-2?
🆚 Go to Comparative Table 🆚The main difference between phosphofructokinase-1 (PFK-1) and phosphofructokinase-2 (PFK-2) lies in their catalytic functions and regulation:
- Catalytic Functions:
- PFK-1 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate, which is the rate-limiting step of glycolysis.
- PFK-2 catalyzes the conversion of fructose-6-phosphate to fructose-2,6-bisphosphate.
- Regulation:
- PFK-1 activity is affected by ATP concentration, while PFK-2 activity is not.
- PFK-2 has both kinase and phosphatase activity, making it a bi-functional enzyme.
- Fructose 2,6-bisphosphate, a product of PFK-2, is a powerful allosteric activator of PFK-1.
In summary, PFK-1 and PFK-2 are both involved in the regulation of glycolysis, but they catalyze different reactions and are regulated differently. PFK-1 is responsible for the rate-limiting step of glycolysis, while PFK-2 plays a role in activating PFK-1 through the production of fructose 2,6-bisphosphate.
Comparative Table: PFK-1 vs PFK-2
The key difference between PFK-1 and PFK-2 lies in their specific roles in the glycolysis pathway and their regulation:
Feature | PFK-1 (Phosphofructokinase-1) | PFK-2 (Phosphofructokinase-2) |
---|---|---|
Function | Catalyzes the conversion of fructose 6-phosphate into fructose 1,6-bisphosphate. | Catalyzes the conversion of fructose 6-phosphate into fructose 2,6-bisphosphate. |
Regulation | PFK-1 activity is affected by ATP concentration. | PFK-2 activity is not affected by ATP concentration. |
Role in Glycolysis | PFK-1 is a glycolytic enzyme and serves as the rate-limiting step of glycolysis. | PFK-2 is not a rate-limiting enzyme in glycolysis. |
Activation | Fructose 2,6-bisphosphate, synthesized by PFK-2, is a powerful allosteric activator of PFK-1. | PFK-2 is not regulated by allosteric activation. |
Both PFK-1 and PFK-2 use the same substrate, fructose 6-phosphate, and add an extra phosphate to the substrate during phosphorylation. However, the products of their reactions and their roles in glycolysis are different.
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